Project Description

reversed phase polymer chromatography

EPRUI Biotech Co. Ltd. offers a new generation of reversed phase polymer HPLC packing material. Our polymer chromatography column packing material is made of highly crosslinked monodisperse polymer resins including highly crosslinked polystyrene divinylbenzen, polymethyl methacrylate, copolymers of PS/DVB and PMMA.

With precise control of particle size, particle size distribution, specific surface area, pore size structure and surface functional groups, our monodisperse polymer HPLC packing material has great properties including high resolution and loading capacity, strong rigidity, acid and base resistant, low back pressure, long lifetime and low non-specific adsorption.

Our polymer based reverse phase chromatography packings have been widely used in high performance liquid chromatography (HPLC) analysis, isolation and purification of organic compounds, proteins, polypeptides, nucleic acids, natural products and synthetic pharmaceuticals.

EPRUI supplies 2um nonporous polystyrene microspheres which is for UPLC application; 5um and 10um porous polystyrene microspheres for HPLC analysis use; 10um, 15um, 30um, 40um, 50um porous porous PS and PMMA microspheres for preparative chromatography use. Pore sizes can be chosen from 100A, 300A, 500A, 1000A which can satisfy various biological purification requirements.

  1. Highly uniform particle size
  2. Perfect spherical morphology
  3. Optimized pore structure
  4. Good chemical and PH stability
  5. High mechanical strength
  6. Scale production

1. Highly uniform particle size and perfect spherical morphology

It is very important for chromatographic application by controlling particle size and monodispersity of microspheres because column efficiency is usually decided by particle size and its distribution. During chromatographic separation, the longitudinal diffusion of solutes is the main reason for broadening of chromatographic bands and peaks. Columns packed with uniform microspheres can effectively narrow the bands and peaks which provide the lowest back pressure and make the columns with high efficiency and high resolution.

Compared with other commercial chromatography media, our monodisperse microspheres have demonstrated great advantages of strong rigidity, easy column packing, low back pressure, high column efficiency, good resolution, stable column bed and lower risk of frit-plugging.


Figue1: Monodisperse Polymer Microspheres with various particle size


Figure2: Particle Size Distribution of 30um Polystyrene Microspheres

2. Optimized pore structure

Pore size and specific surface area have a strong influence on the separation performance of chromatography media. The choice of a chromatography media with correct pore structure would improve the loading capacity, the column efficiency and the final purity of target molecule. We offer media with pore sizes typically from 50Å up to 1000Å.


Figure3: Polymer microspheres with different pore size


Figure4: Comparison of separation result with different pore size

Samples:Ribonuclease B, Recombinant human insulin, Cytochrome C, Lysozyme,  BSA
Mobile Phase A:0.1% TFA in water
Mobile Phase B:0.1%TFA in acetonitrile
Gradient:20%B to 40% B,20 min; 40% B,10 min


3. Superior chemical and pH stability

Reverse Phase PS/DVB V.S.  Silica Gel C18

The biggest advantage of highly cross-linked PS/DVB chromatography over silica gel chromatography is its excellent pH stability over a wide range of pH 1-14. Polymer chromatography media maintain their outstanding physical and chemical stability even in extreme acid or alkaline solutions (for e.g. 1 M NaOH/HCL) and organic solvents (such as methanol, ethanol, acetone, n-propanol, isopropanol, DMSO, THF, acetonitrile, 6 M guanidine hydrochloride, etc.). Besides, EPRUI provides the customers a wider choice of conditions for process development and optimization. The outstanding chemical stability, have been proven to meet the requirements of Cleaning-in-Place (CIP) in cGMP operations. For example, the loading capacity and separation efficiency remain unchanged, after 10um 300A PS/DVB column was soaked in 1 M NaOH at 60°C for 40 days (Figure 1, used for Insulin purification).


Figure 1:Insulin loading capacity when EPRUI-PSD10-300 column was soaked in 1M NaOH (at 60℃) for 40 days.

4. Mass production and Great batch reproducibility

Our advanced manufacturing facilities and  a precise quality control system guarantee consistent high quality and batch-to-batch reproducibility of the bulk production of media. Figures below show the results of the performance test of more than twenty different lots of the chromatography media, indicating the good batch-to-batch reproducibility in our production.


Figue5:Consistent Particle Size Distribution—30um


Figue6:Consistent Particle Size Distribution—40um


Figure7: Stable pore volume and specific surface area

Figure8: Reproducible isolation of four proteins by 18 lots

chromatographic column:4.6 mm I.D. x 250 mm, EPRUI- 40PS-300
Mobile Phase A:0.1% TFA in water
Mobile Phase B:0.1%TFA in acetonitrile
Gradient:20%B to 40% B,20 min; 40% B,10 min
Flow Velocity:1 ml/min;80%B to 95%B,10min
Wavelength:UV @ 280nm

Our monodisperse polymer microspheres have been successfully applied in the isolation and purification of plant extracts, antibiotics, polypeptides and others.

Plant ExtractsAntibioticsPolypeptidesOthers
DocetaxelDerivatives of vancomycinThymalfasinIopamidol
NeomangiferinPneumocandin B0

Purification Case of Insulin

EPRUI develops a whole set of insulin purification technology which can successfully improve the purity of insulin from 45% to 99%



Instrument: High pressure equipment

Loading Amount: 100mg/ml

Test: 214nm

ItemParticle SizePore SizeMatrix
EPRUI-PSM1010umNonporous,100,300,500,1000APoly DVB/acrylate
EPRUI-PSM1515umNonporous,100,300,500,1000APoly DVB/acrylate
EPRUI-PSM2020umNonporous,100,300,500,1000APoly DVB/acrylate
EPRUI-PSM3030umNonporous,100,300,500,1000APoly DVB/acrylate
EPRUI-PSM4040umNonporous,100,300,500,1000APoly DVB/acrylate
EPRUI-PSM5050umNonporous,100,300,500,1000APoly DVB/acrylate
EPRUI-PSM100100umNonporous,100,300,500,1000APoly DVB/acrylate
EPRUI-PSM200200umNonporous,100,300,500,1000APoly DVB/acrylate