Solid Phase Extraction Chromatography

>Solid Phase Extraction Chromatography
Solid Phase Extraction Chromatography 2018-04-03T07:52:27+00:00

EPRUI Biotech Polymer Microspheres for SPE:

  • Completely Waters Oasis HLB or Phenomenex Strata X Replaceable
  • Unbeatable Favorable PRICE!

Introduction of Solid Phase Extraction (SPE)

Solid phase extraction (SPE) is a sample preparation process by which compounds that are dissolved or suspended in a liquid mixture are separated from other compounds in the mixture according to their physical and chemical properties.

When a sample passes through the extraction column filled with a sorbent, a part or all of the constituents interact with the sorbent and are retained in the column. A solvent or mixture of solvents is then used to wash out interferences. The target analyte is then quickly eluted out of the column by a desired solution of reduced volume. The goal of fast separation, purification, and concentration is therefore achieved. SPE can be carried out with various types of sorbents to perform extraction and separation of different analytes. Depending on the nature of the sorbents, SPE extraction materials can be divided into normal phase sorbents, reverse phase sorbents and ion exchange sorbents.

The first generation of SPE used bonded silica gel as the base sorbents. As technology evolves, polymer microspheres for SPE sorbents are gaining popularity and acceptance because of the shortcomings of bonded silica such as poor pH stability and low absorption capacity.

EPRUI Biotech supplies monodisperse polymer sorbents which has a large surface area and high loading capacity of functional groups on the particles. The Coefficient of Variation (CV) of the polymer microspheres is less than 3%, which provides outstanding recovery rate , high loading capacity and selectivity, good reproducibility and elution concentration plus a reduction in solvent usage.

Product List

2018-04-01T15:59:21+00:00

Anion Exchange Chromatography

SPE-SAX and SPE-WAX are used for the isolation and purification of acidic compounds in basic or neutral medium. Based on a mixed mode of anion ion exchange and hydrophobicity interaction with functional quaternary / tertiary amino groups, SPE-SAX and SPE-WAX can quickly and steadily recover acidic compounds from the [...]

2018-04-09T15:28:16+00:00

SPE HLB Sorbents

SPE-HLB sorbents are produced with a special polymerization technique. It is corrresponding to Water Oasis HLB or Phenomenex Strata X. They are reverse phase sorbents wettable by water with balanced hydrophilicity and hydrophobicity capable of satisfying most solid phase extrction needs. Monodisperse microspheres in SPE-HLB sorbents are made with outstanding [...]

2018-04-01T13:26:01+00:00

Cation Exchange Chromatography

SPE-HLB sorbents are produced with a special polymerization technique. It is corrresponding to Water Oasis HLB or Phenomenex Strata X. They are reverse phase sorbents wettable by water with balanced hydrophilicity and hydrophobicity capable of satisfying most solid phase extrction needs. Monodisperse microspheres in SPE-HLB sorbents are made with outstanding [...]