Project Description

amino magnetic bead

Amino Magnetic Beads are superparamagnetic beads with rich amino groups on the surface thus can bind to polypeptides, antibodies and other proteins, oligonucleotides and certain chemical compounds with certain chemical reagent (such as glutaraldehyde). Amino magnetic nanoparticles are widely used as an important carrier of the medical and molecular biology research tools.

EPRUI Biotech supplies two types of amino treated magnetic microspheres (EPRUI-MagNH2): ① amino functionalized magnetic silica beads ② amino treated magnetic polystyrene microspheres. Our amino magnetic beads are with higher magnetic response, lower nonspecific adsorption and richer binding sites with activated groups of amino on the surface, which can easily and efficiently combine with a variety of ligands such as protein, polypeptide, oligomeric nucleotide and drug molecules, especially for batch isolation and purification.

① Amino functionalized magnetic silica beads

amino treated magnetic silica beads

Amino functionalized magnetic silica beads have the same structure as silica coated magnetic beads. It is also with typical core shell structure. The core is superparamagnetic Fe3O4 nanospheres which is coated with silica layer as shell. Amino groups are from surface modification by KH550. Besides amino groups, there are still some –SiOH groups left on the surface. Therefore, amino functionalized magnetic silica beads are not suitable for separation and purification of protein as well as other biomacromolecule. It can be widely used to:

  1. Nucleic acid separation
  2. Enzyme fixation
  3. Catalyst carrier
  4. Separation and purification of small molecules

② amino treated magnetic polystyrene microspheres

amino functionalized magnetic polymer beads

The structure of amino treated magnetic polystyrene microspheres is typically sandwich type. The inner core is monodisperse polymer microspheres. Magnetic oxide layer is in the middle between uniform polymer microspheres and the outermost layer which is hydrophilic and hydroxyl functionalized polymer layer. By amination, hydroxyl groups convert to amino groups.

Through cross-linking reagents (such as glutaraldehyde, etc.), amino treated magnetic polystyrene microspheres are able to covalently coupled with protein ligands (such as antigen, antibody, etc.), oligonucleotide probes and other biomolecules on the surface,which makes amino treated magnetic polystyrene microspheres an important carrier in medical and molecular biology research.

It can be widely used as below:

  1. Nucleic acid separation
  2. Immunoassay
  3. Cell separation
  4. Biosensor
  5. Drug combination and targeted delivery

 

  1. Narrow particle size distribution
  2. High reproducibility
  3. Good biocompatibility
  4. Superparamagnetic and high magnetic response(Saturation magnetization is greater than 40emu/g ), saving operation time.
  5. Very rich binding sites and biological ligand coupling sites.
  6. Good dispersion effect, suitable for automation

Amino functionalized magnetic silica beads Amino treated magnetic polystyrene microspheres
  1. Nucleic acid separation
  2. Enzyme fixation
  3. Catalyst carrier
  4. Separation and purification of small molecules

 

  1. Nucleic acid separation
  2. Immunoassay, can be functionalized with protein and purify antibody
  3. Cell separation
  4. Biosensor
  5. Drug combination and targeted drug delivery
  6. Detecting biologically active substances
  7. Sample enrichment and separation

Brand Product Name Code No. Particle Size Functional Groups Core Shell Solvent Recommended Buffers
EPRUI-MagNH2 Amino functionalized magnetic silica beads 120020 200nm -NH2 Fe3O4 SiO2 DI Water PBS, Tris-HCl and H2O
120100 1um
120300 3um
120500 5um
121100 10um
Amino treated magnetic polystyrene microspheres

 

130020 200nm -NH2 Polymer/Fe3O4 Polymer DI Water PBS, Tris-HCl and H2O
130100 1um
130300 3um
130500 5um
131100 10um

How to Use:
Use a mixer that provides rotation of the tube to ensure that the magnetic beads do not settle in the tube before use. Avoid air bubbles during pipetting.
Storage & Shelf Life:
Store at 4-8℃. Do not freeze or dry into powder. 12months