Project Description

carboxyl magnetic beads

Carboxyl Magnetic Beads are superparamagnetic beads with high carboxyl content on the surface thus can bind to polypeptides, antibodies or other proteins, oligonucleotides and certain chemical compounds with certain chemical reagent (such as EDC). They are widely used as an important carrier of the medical and molecular biology research tools.

EPRUI Biotech supplies two types of carboxyl treated magnetic microspheres (EPRUI-MagCOOH): 1. carboxyl functionalized magnetic silica beads 2.   Carboxyl treated magnetic polystyrene microspheres. Our carboxylized magnetic microspheres have higher magnetic response, lower nonspecific adsorption and richer binding sites with activated groups of carboxyl on the surface, which can easily and efficiently combine with a variety of ligands such as protein, polypeptide, oligomeric nucleotide and drug molecues, especially for batch separation and purification.

① Carboxyl functionalized magnetic silica beads

carboxylized magnetic silica beads

Carboxyl functionalized magnetic silica beads have the same structure as silica coated magnetic beads. It is also with typical core shell structure. The core is superparamagnetic Fe3O4 nanoparticles which is coated with silica layer as shell. Carboxyl groups are from surface modification of amino treated magnetic silica microspheres. Besides carboxyl groups, there are still some –SiOH groups left on the surface. Therefore, carboxyl functionalized magnetic silica microspheres are not suitable for separation and purification of protein as well as other biomacromolecule. It can be widely used to:

  1. Nucleic acid separation
  2. Enzyme fixation
  3. Catalyst carrier
  4. Separation and purification of small molecules

②Carboxyl functionalized magnetic polystyrene beads

carboxyl treated magnetic polystyrene beads

The structure of carboxyl treated magnetic polystyrene microspheres is typically sandwich type. The inner core is monodisperse polymer microspheres. Magnetic oxide layer is in the middle between uniform polymer microspheres and the outermost layer which is hydrophilic and hydroxyl functionalized polymer layer. By carboxylation, hydroxyl groups convert to carboxyl groups.

Through cross-linking reagents (such as EDC etc.), amino treated magnetic polystyrene microspheres are able to covalently coupled with protein ligands (such as antigen, antibody, etc.), oligonucleotide probes and other biomolecules on the surface,which makes amino treated magnetic polystyrene microspheres an important carrier in medical and molecular biology research.

It can be widely used as below:

  1. Nucleic acid separation
  2. Immunoassay
  3. Cell separation
  4. Affinity chromatography and purification

  1. Narrow particle size distribution
  2. High reproducibility
  3. Good biocompatibility
  4. Superparamagnetic and high magnetic response(Saturation magnetization is greater than 40emu/g ), saving operation time.
  5. Good dispersion and resuspend, suitable for automattion
  6. Great target binding capacity, low non-specific adsorption: dedicated to the special nucleic acid isolation and protein purification.

Carboxyl functionalized magnetic silica beads

 

Carboxyl functionalized magnetic polystyrene beads

 

1.    Nucleic acid separation

2.    Enzyme fixation

3.    Catalyst carrier

4.    Separation and purification of small molecules

1. Nucleic acid separation

2. Immunoassay

3. Cell separation

4. Affinity chromatography and purification

5. Protein Purification

6. Biological sensor

7. Drug screening and delivery

Brand Product Name Code No. Particle Size Functional Groups Core Shell Solvent Recommended Buffers
EPRUI-MagCOOH Carboxyl functionalized magnetic silica beads

 

140020 200nm -COOH Fe3O4 SiO2 DI Water PBS, Tris-HCl and H2O
140050 500nm
140100 1um
140300 3um
140500 5um
141100 10um
Carboxyl functionalized magnetic polystyrene beads

 

150020 200nm -COOH Polymer/Fe3O4 Polymer DI Water PBS, Tris-HCl and H2O
150050 500nm
150100 1um
150300 3um
150500 5um
151100 10um

Storage & Shelf Life:
1. Store at 4-8℃. Do not freeze or dry into powder.
2. 12 months