Gel permeation chromatography (GPC) is a type of size exclusion chromatography (SEC), that separates analytes on the basis of size. It is often necessary to separate polymers, both to analyze them as well as to purify the desired product. GPC systems and consumables are now also available from a number of manufacturers.
EPRUI Biotech has developed monodisperse gel microspheres used for gel permeation separation of lipophilic polymers and hydrophobic molecules in the presence of organic solvents. The matrix of our gel permeation chromatography is styrene and divinylbenzne copolymer, which is nonporous transparent beads in dry form and will become porous network structure which has molecular sieve property after swelling in organic solvents. When the gel beads completely swell, the pore size or exclusion limits depend on the crosslinking level and eluant employed.
EPRUI GPC media are available with crosslink degree from 1-12% and have exclusion limits ranging from 400-14000 daltons. Compared with other GPC media in the market, our monodispersed gel beads with precisely controlled diameter offer high separation resolution of lipophilic polymer and oligomers, low back pressure and minimal elution volume. The monodisperse polystyrene microspheres typically swell in benzene, toluene, xylene, carbon tetrachloride, cyclohexane, tetrahydrofuran, dimethylformamide, ketones, methylene chloride and other mixtures of solvents. Complete swelling is necessary before packing in the column and running the chromatography applications.