Protein A Affinity Chromatography daphne 2017-03-06T14:22:23+00:00
Protein A affinity chromatography medium by EPRUI is based on the monodisperse porous poly (methyl methacrylate) (PMMA) microspheres as the matrix, and genetically modified protein A as the ligand, which ensures its high affinity binding of monoclonal antibodies and Fc fragment containing recombinant proteins (macromolecules). Our protein A chromatography media has high mechanical strength and good pH stability. Even at a high flow rate, the beads still maintained a high dynamic binding capacity, meeting the demands from laboratory preparation to industrial production.
1. Highly uniform particle size EPRUI protein A affinity chromatography medium has highly uniform particle size distribution and precisely controlled pore structure, as shown in Fig. 1.
2. High flow rate EPRUI protein A affinity chromatography media is made of polymethyl methacrylate (PMMA) microspheres. Our protein A media is able to withstand pressure of 0.5 MPa. Compared with the affinity medium with conventional alagarose matrix, EPRUI protein A will make the separation process much faster, therefore reducing the purification time. It is ideal chromatography media for hundreds of liters of large scale chromatography preparative column. Experimental：Column：4.6 mm × 50 mm Mobile phase：50 mM PBS，0.5 M NaCl
Figure 2. Comparison of EPRUI protein A media and a conventional agarose type protein A medium pressure vs. linear flow rate.
3. Excellent loading capacity At a high flow rate, EPRUI protein A media shows a much better dynamic binding capacity (DBC) than that of the conventional agarose type protein A medium. Even when the residence time was increase to 4-6 minutes.(Experimental：Column：7 mm × 25 mm Sample：Human IgG，2 mg/mL Equilibration buffer：20 mM PBS，pH 7.0 ，0.15 M NaCl Elution：0.1 M Glycine ，pH 3.0)
Figure3. Comparison of EPRUI protein A media with a leading brand of agarose type Protein A medium for their dynamic binding capacity
4. Outstanding pH stability EPRUI protein A affinity chromatography medium could be applied in a pH range from 3-12, and it could be cleaned with 0.1-0.5 M NaOH. In a life-cycle test,EPRUI protein A medium remains more than 90% of the original dynamic binding capacity (DBC), after 100 CIP cycles with 0.5 M NaOH as CIP reagent. (CIP procedure：Medium：Uni®Mab Column：7 mm × 25 mm Sample：recombinant antibody(chimera) DBC calculation：10% breakthrough Equilibration buffer：20 mM PBS，150 mM NaCl， pH 7.2，3 CV（column volume) Elution：20 mM Citrate，pH 3.0 CIP：0.5 M NaOH，15 CV Re-equilibration：10 CV)
Figure 4.EPRUI protein A media pH stability in life cycle tests